The reconstructed human epidermis expresses the major differentiation markers (filaggrin and involucrin in granular cell layers, transglutaminase I and keratin 10 in supra basal cell layers and loricrin in upper granular cell layers), as well as expressing the basement membrane markers (type IV collagen; integrin alpha 6, integrin beta 4, antigen BP, laminin I and laminin V).
Free fatty acids and ceramides are detected in the lipid profile. The ultra-structural features show secretion and normal arrangement of bi-layered lipid content into the intercellular spaces of the cornified cell layers (formation of normal permeability barrier).