SkinEthic Labotories

Characterization


Keratin detection
Keratin detection


CD 44 detection
CD 44 detection

The reconstructed human corneal epithelium secretes the same mucins that are being found in the human cornea in vivo. Ultra-structural results show the typical presence of a columnar basal cell layer, 2-3 layers of transitional wing cells, and 2-3 layers of superficial squamous cells.

 
 
In vivo hemidesmosomesIn vitro reconstituted human corneal epithelium: presence ofhemidesmosomes


Hemidesmosomes and associated anchoring filaments form the complex for the firm attachment of the epithelium to the stroma.

High-power transmission electron micrograph of the basal membrane of a basal cell revealing numerous mature hemidesmosomes. Also visble are the components of the hemidesmosomes: the intracellular membrane placode, filaments radiating through the membrane, and the typical band in what would be the lamina lucida. The amorphous band at the bottom is the polycarbonate support membrane of the construct. Original magnification 75,000x.